Clinical tests and animal experiments

Survival rate of Caenorhabditis elegans

吉報源による線虫の生存率

According to cytobiology, Caenorhabditis elegans has the same gene structure as human beings. Its life span is 3 weeks, and in 10 days, it becomes middle aged and its life exhausts afterwards which is the reason Caenorhabditis elegans are recently being used in many experiments and tests.

We have seen 35% of prolonging of the life span in the nematodes from the influence of Kippogen. The average life-span of the C. elegans is 21 days, and after administering Kippogen the survival of the senile nematodes born after 17 days (in human being relevant to 100 years old) was 80%, and the survival rate of the control groups was that of 20%.

Findings suggest that one can live up to 100 years without care and pass away.
Conclusion of this test
Prolongs the life-span. We were able to confirm up to maximum of 35% in life extension.
Change in physiology activation. Increase of ATP activity. Restrains the growth of SOD synthesis and alleviates the strain of Caenorhabditis elegans.
ATP is the start of all life activities. It is the origin of all growth hormones, insulin and enzymes. Consuming bread or rice would convert into ATP and be consumed within the body. Heart movement, conversing, movement as walking, these all come from the energy source. ATP cannot be saved and stored, and the stop of production of the ATP means death.

SOD enzyme has the factor to eliminate active oxygen. Normally when ATP is activated, the active oxygen will increase, but there is a result that these two have been restrained at the same time. The restriction of both ATP activity and active oxygen at the same time is a result that can be said as vice versed.
Light diet is recommended for the elderly to restrain active oxygen, but this result shows totally of the opposite. Another observation seen is that when the Caenorhabditis elegans age and their sights weaken, they are still able to move around actively.
Incidentally, 74% of the Caenorhabditis elegans gene structure is the same as of humans and these test results may be said for the human beings also.

[Test A]Anticancer effect in liver cancer mouse

We have tested the Colon26 cancer mice by giving them Kippogen for 14 consecutive days and the control group without Kippogen, and compared the cubic volume of the cancer. The volume of the tested group was approximately 50%, only half the size of the other. The concentration of the tumor necrosis factor called TNF-a cytokine (immune) was double in the Kippogen applied mice group.


[ Comparison of cancer tumor volumes ]
tumor volumes (mm3)
Control mouse 626.38
KIPPO mouse 330.02


[ concentration of IL-12 & TNF-α ]
concentration of IL-12 & TNF-α (pg/ml)
IL-12 TNF-α
Control 1.96 20.45
KIPPO 2.11 37.46

[ Observation ]
It is considered that by administering Kippogen, the growth of the tumor cells have been restrained by the elevation of TNF-a production. One example is, a stage 3 breast cancer patient with leukocytosis, WBC over 12,000/?, the number has leveled to a normal standard in half an year.(Exactly when is unknown)

In case of lacking white blood cells, they will elevate and become normal. Kippogen has immune regulatory function. Neither less to say, Kippogen acts as a precursor, so synthetic TNF will not be detected.

Function of TNF-a
◆ Activation of white blood cell ◆ Production of B cell antibody
◆ Production of cytokine macrophage ◆ T cell interleukin-2 (IL-2)
◆ Marrow neutrophile ◆ Growth of fibroblast
◆ Osteoclast activation ◆ Generates osteoblast enzymes
◆ Restrains tumor cell ◆ Releases muscle amino acid
◆ Binds neutrophile cytokine generation ◆ Brain Fever NREM sleep Intravascular cell

[Test B]Fluctuation of lipid peroxide concentration within the blood in case of aerobic exercise.(Assuming anticancer therapy)

We administered Kippogen to mice for 3 consecutive days, and had them do aerobic exercise to see the change of lipid peroxide concentration within the blood generated from stress. As for the control group, the lipid fat concentration within the blood right after the stress exercise was 3.3nmol/ml, 30 minutes later 4.4nmol/ml. Comparing to the Kippo group, the number was 2.0nmol/ml right after the stress exercise, and 2.2nmol/ml 30 minutes later which showed that the number was lower relative to the one not taking the Kippogen.
These results have proven that by administering Kippogen, it is able to restrain the hyper oxidation of the lipid peroxide occurred from aerobic exercise.


[ Table 1 Concentration of lipid peroxide in case of aerobic physical movement ]
lipid peroxide concentration within the blood (nmol/ml)
0min 30min
Control 3.3 4.4
KIPPO 2.0 2.2
[ Observation ]
As a main function, Kippogen has shown to reduce the side effect caused from chemotherapy and radiation therapy, concurrently 50% of active oxygen generated exhaust fume and from smoking.

Another action implied is once the active oxygen generates, it will take 6 hours for the chain reaction to seize, and during that time the white blood cells immune system declines, but it showed that the chain reaction is restrained by taking Kippogen.

[Test C]Fluctuation of lipid peroxide concentration when put under stress.(Assuming anticancer therapy)

After 3 consecutive days of giving Kippogen, we have put the mice under air jet stress and measured the stress secreted from the brain of hyperoxidation of lipid peroxide within the blood. The control groups number of the hyperoxidation of lipid peroxide put under stress right after was 1.8nmol/ml, and 30 minutes later the number was 0.9nmol/ml. Group that was given Kippogen was 0.9nmol/ml, and after 30 minutes, 1.2nmol/ml which shows that the comparative group had a lower rate.

These results confirmed that by taking Kippogen, it is possible to restrain the hyperoxidation of lipid-fat generated when put under stress.


[ Table 1 Concentraion of the lipid peroxide within the blood when put under stress ]
Lipid peroxide concentration (nmol/ml)
0min 30min
Control 3.3 4.4
KIPPO 2.0 2.2

[Test D]Test of high-fat food using rat

The rat was divided to a group of Kippo + high fatting food, and that of high fattening food only and Kippo group was given 8% more food than the other. After 3 months, the weight of the Kippo group rat was 5% lower compared to the other, even if they had consumed 8% more food.

[Test E]Test of anti cancer cell using liver cancer cell.

The following photograph is the cell of the liver cancer before processing it with Kippogen. 3 days after treated with Kippogen, we have been able to observe that the carcinoma cell has rounded and died.